The present research was focused on probiotic characterization of lactic acid bacteria from soil and fermented foods.L acetic acid bacterial isolates were examined in-vitro for their probiotic characteristics and their antimicrobial activities against some human pathogens. For potential probiotic properties based on their Low pH tolerance, Bile salt resistance, Production of an antimicrobial substance, Proteolytic activity. The result shows that isolates belonged to the genus Lactobacillus. LAB isolates L5, L1 and L10 showed high acid tolerance at pH3.0 and pH2.0 for 3hrs, 0.3% Bile salt for 4hrs.13. They were exhibited good ability to attach to intestinal epithelial cells and were not resistant to the tested antibiotics they also showed good. Antimicrobial activities against the tested pathogenic strains of human and most of them exhibited stronger antimicrobial activity than the reference strain L. casei Shirota. Therefore, an isolated strain is thought to survive through the intestinal ecosystem and is considered to be suitable as a bio preservative and can be used as a development of various probiotic food products.