Regeneration is repair and regrowth of the lost tissues or organs in response to injury. To unravel the process of regeneration, it is really important to understand the repair mechanism and proteins involved in the process of tissue repair as well as regrowth, factors affecting them and the regulation of those factors. The present study was conducted to firstly observe the modulation of those proteins using different pharmacological agents to check on the regenerative ability of Eisenia fetida by targeting oct ¾, Nanog and lin28. These genes and their regulatory product proteins respectively were found to be modulated using pharmacological agents like isotroin and Metformin. The other proteins which were involved in the regeneration process were expected to get illuminated in protein profiling on using these agents. By using Metformin and Isotroin which were involved in the regulation of proteins like oct-4 and lin-28 which were themselves involved in regeneration, we could detect the upregulation rather the complete production of other proteins like lumbrokinase, gag, CRT which were concluded to be responsible for the regeneration in Eisenia fetida.