Three new monocot lectins from the members of Zingiberaceae, namely, Alpinia galanga L. (AGTL), Curcuma amada Roxb. (CARL) and Elettaria cardamomum M. (ECSL) have been purified by sepharose and agarose affinity chromatography. These lectins possess similar physicochemical and biological properties. In SDS-PAGE, all the lectins gave a single band corresponding to a subunit of 16 and 17kDa and yielded single band peak on PAGE (pH 4.5). The lectins agglutinate rabbit and sheep red blood cells (RBCs) but are inactive towards chicken and human ABO erythrocytes. The haemagglutination activity of these lectins is inhibited by mannose and their derivatives while other simple sugars D-galactose, D-maltose, D-fructose, sucrose, lactose, D- sorbitol, D-mannitol did not react. The lectins activity were enhanced at acidic but stable at physiological pH range of 6- 8. Lectins were stable at 50°- 60° C but able to agglutinate for a short period when it increases.