Ammonia removal from wastewater has become a worldwide emerging concern as ammonia proves to be toxic to aquatic species and also causes eutrophication in the natural water environment. Nitrogen compounds in our effluent have been removed by means of biological approaches(micro-organisms). Based on the microbial nitrogen cycle many biological technologies and processes have been developed and implemented for nitrogen removal from wastewater, such as de-nitrification, nitrification and by the use of biological species. However, with the effluent discharge standards have become more stringent (<10mg total nitrogen/L), conventional processes cannot meet the new requirements. We have removed the amount of nitrogen with the help of bacteria such as Nitrosomonas, Nitrospira. The limit of discharging nitrogen is 50ppm into the rivers. So by using these bacteria, our aim will be fulfilled.