ABSTRACT Safe motherhood care is the right of every woman, and it is the responsibility of the welfare state to provide quality maternal health services. Respectful maternity care (RMC) is not only a quality indicator of maternity care, but it also assures that all childbearing women's essential human rights are protected. Methods: A cross-sectional study was conducted to assess the Respectful Maternity Care Practices during labor among 60 nursing personnel from maternity centers who were selected by consecutive sampling technique. The data was collected by observation method, using pretested and validated tools such as background variables proforma of nursing personnel and standardized white ribbon Alliance Charter on Respectful Maternity Care during labor. Results: The findings revealed that 96.7% of the nursing personnel exhibited moderately adequate practice in respectful maternity care. The global mean practice score was 30.60/52 ± 2.76 (58.84%). There was a significant association between selected background variables—age, years of experience, marital status, number of children—and the practice of nursing personnel on respectful maternity care (RMC) during labor (p < 0.05).Conclusion: Implementation of Respectful Maternity Care needs and change of attitudes of nursing personnel who render care during childbirth will address the issue of disrespect and abuse towards women and provide happy and fulfilling experiences for all women during labor.