Agriculture is the only basis for feeding humanity. Adoption of modern agricultural practices and use of technology is inadequate in India. Arachis hypogea L. (Groundnut) belongs to family Fabaceae. It is an annual. The roasted seeds are edible. Oil is extracted from the seeds and used as fine cooking medium. Vegetable ghee (peanut butter) is also prepared from this oil. The oil cake is fed to the livestock. It is rich in fatty acids and proteins. The major constraints of Groundnut production in India are unreliable rain fall pattern, pest and diseases, socio-economic status of farmers, failure of communication media to reach in the area, lack of awareness and traditional mind psychology of farmers about improved agro-practices (Baldeo Sigh 1990). The chemical fertilizers cause impact on soil micro flora and fauna. In such situation it is need of the day to develop new easy low cost eco-friendly Rhizobium bio-fertilizer technology for Groundnut and residual effect of Rhizobium culture on yield of wheat, rice or subsequent crop etc. is always higher (Raverkar, K. P. and Konde, B. K. 1988). Present research work shows application of Rhizobium bio-fertilizer technology for Groundnut seeds and to study the increase in yield of pods during the crop season 2018. It was observed that, the yield increases by 20 to 25 %.