Now a days the box girder bridge is frequently used bridge system in worldwide. Actually, the box girder bridge is commonly used for the long span for the ignoring the heavy weight as compare to the other bridge girder system. CSiBridge v 20.0.0 is used for analyzing dynamic response of box girder bridge. The main objective of this study is analyzing and investigating seismic performance of multi-span with up to five spans 92-meter-long box girder bridge. The seismic performance of box girder bridge is very complex and performance depends on the peak ground motion and, ground motion acceleration. It is done by non-linear time history analysis method. In this study, the three-dimension model and the data of Kobe earthquake are used for dynamic characteristics and showing the maximum response of box girder deck. Response results indicate in terms of deformed shape, absolute acceleration, base shear, base reaction, total energy component and displacement. Thus, the paper suggests that the construction of bridge based on time history analysis will sustain the earthquake up to 6.9 magnitude.