The Atrazine and Terbuthylazine molecules are being used alone and a combination as a special herbicide activity to control the broadband leaves in the agricultural industry. Atrazine and Terbuthylazine have a high capillary action in the roots of brad spectrum plants with respect to other herbicide molecules. The solubility of Atrazine and Terbuthylazine also very high solubility in water and this solubility enhanced the capillary action through the broad leaves plant roots. The persistence of this Atrazine and Terbuthylazine is very long period hence the residue levels in the used substrates are being existed in the used substrates. Even though the molecules Atrazine and Terbuthylazine are less toxic to humans and animals, the lowest detection levels have to be determined with a simple HPLC analytical method; which is very effective time and cost of analysis. Within 10 minutes of analysis time these two molecules to be determined by using acetonitrile and water as a mobile phase with a ration of 80:20 (volume/volume) with the help of Qualis BDS C18 (250 x 4.6, 5μ) HPLC column at 1 ml/min. flow rate. The detection wavelength is 220 nm by a Shimadzu LC2030 model HPLC. The results of the analysis deliver that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the identification and quantifications of these molecules interims of validation parameters viz., separation, system suitability, System Precision and linearity in a simple HPLC analysis.