Structural analysis is the process of studying the effects of loads on the physical structure and its components. Single Stroke engine consists of a piston, a connecting rod, a crankshaft, and one cylinder. This paper contains the static structural analysis of a Single Stroke Engine and its components i.e., Piston, crankshaft, and connecting rod. Single Stroke engine is mostly used in motorcycles, motor scooters, go-karts, all-terrain vehicles, radio-controlled vehicles, portable tools, and garden machinery (such as lawnmowers, cultivators, and string trimmers). Single Cylinder engines are simpler and more compact when compared to multi-cylinder engines. Effects of loads and different boundary conditions are observed on the single stroke engine using ANSYS software. Ansys Mechanical APDL suite is used for static structural analysis. The material used in this analysis for the Single Stroke Engine is Structural steel. The aim of this paper is to determine the total deformation, Equivalent stress, Equivalent strain on the single stroke engine and its components. Modeling and assembly of parts are done on CATIA V5R21.