The emergence and spread of antibiotic-resistant bacteria in the environment is a serious medical and environmental problem worldwide.This study aimed to assess the microbiological quality and antibiograms of bacterial isolates from water bodies in Chennai , India to assess the prevalence and antibiotic resistance profiles of these bacteria in environmental samples .Antibiotic-resistant bacteria are able to transfer their genes to other aquatic bacteria, which thus acquire new resistance genes Isolating bacteria from clinical or environmental samples involves specific microbiological methods such as aiding in diagnosis, and guiding effective treatment strategies in clinical and research settings.Water samples were collected from various sources, including rivers, lakes, and reservoirs, and bacteria isolates were identified and characterised using standard microbiological methods. drinking water coming from these sources contains microorganisms Most often, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterobacter aerogenes, Klebsiella spp.Salmonella typhi Were sown from water samples. Studies have shown that all isolated microorganisms have multi-resistance to one degree or another. But Escherichia coli and Klebsiella pneumoniae had the greatest resistance to various antibiotics.