Nanotechnology is an important field of modern research dealing with design, synthesis, and manipulation of particle structure ranging from approximately 1-100 nm. Silver nanoparticles are of interest because of its unique properties as they hold the acceptance to kill microbes effectively. The synthesis of silver nanoparticles in the green process is a cost-effective and environment friendly technique from 1mM silver nitrate solution through the extract of Citrus maxima. The change of color indicates the syntheses of nanoparticles. Nanoparticles were characterized using various characterization techniques. This green synthesis provides an economic, eco-friendly, and clean synthesis route to AgNPs. AgNPs in suspension showed activity against Gram-negative and Gram-positive bacteria with minimum bactericidal concentrations (MBCs). The size of synthesized nanoparticle is about 9 nm. Efficient antimicrobial activity of the synthesized silver nanoparticles proves the application potential of green synthesis in the area of nano-medicine.