This research highlights the synthesis of glycoconjugates and recent progress in the development of glycosylated derivative therapeutics. This study lies in the discussion of recent mechanistic theories and supporting experimental evidence on chemical glycosylation on compounds. Here synthesized 10 novel glycosylated derivatives of Thiourea. Glycosylation reaction is achieved by using D-glucose and D-fructose. They were characterized by elemental analysis, IR, and 1HNMR spectroscopies. All the synthesized derivatives were screened for in-vitro antibacterial activity against Bacillus subtilis, Staphylococcus aureus, Escheria coli, and Pseudomonas aeruginosa.