A method has been developed for the quantitative adsorption, of Cu (II)and its separation from other elements.Spectrophotometric determination of Cu(II) has been carried by employingtetraethylenepentamine (TEPA)as a spectrophotometric reagent. Stannic oxide has been prepared by co-precipitation method which is nanomaterial. Stannic oxide is found to be stable towards acids, bases and most of the common chemical reagents. The effects of different parameters such as pH, time of contact, amount of stannic oxide in the adsorption of 50.0µg of Cu (II) has been studied. It has been observed that 250 mg of stannic oxide is sufficient for maximum adsorption (94.34 ± 1.34%) of 50.0µg Cu (II) at pH 5.0 and contact time of 5.0 minutes. Under the optimum conditions of adsorption, effect of various anions and cations in the adsorption of Cu (II) also has been studied. Interfering cations has been masked by using suitable masking agents so as to make the process more selective. Distribution co-efficient values has been determined for various cations under experimental conditions which enable to predict affinity of various cations for the adsorption on stannic oxide.