Air pollution is one of the serious problems faced by people globally due to its transboundary dispersion of pollutants over the entire world. But, whatever mode, whether natural or artificial, is a major concern in to-days developing countries like India. The present research is aimed at assessing the air pollution tolerance index of plants at two different locations. The locations selected were Maheshwari Udayaan, Mumbai, India (located at the center of four signals routes (location 1), and Khalsa College garden, Mumbai, India (location 2). Plants available commonly in both locations were selected for the present research. Four physiological and biochemical parameters which are relative water content, leaf pH, Ascorbic acid, and total chlorophyll were used to compute the APTI. Plants' responses towards air pollution are assessed by the air pollution tolerance index (APTI) value. The plant species having higher APTI value can be given priority for plantation programs in urbanize and industrial areas; so as to reduce the effects of air pollution and to make the ambient atmosphere clean and healthy. The present study was conducted for evaluating the Air Pollution Tolerance Index (APTI) value of six plant species i.e., 1. Mangifera Indica 2.Polyathia longifolia 3. Ixora 4. Codiaeum variegatum 5.Canna Indica 6.Phyllanthus amarus.