Introduction: Bio-Medical Waste Management is one of the biggest challenges of the present-day time because it has a direct impact on the health of the human being. Awareness about various aspects of Bio-Medical Waste Management is required and is needed to be assessed among the health care workers. Objective: To evaluate the effectiveness of Video Assisted Teaching Vs Demonstration Method of Teaching regarding knowledge on Bio-Medical Waste Management and to correlate between the Video Assisted Teaching and Demonstration Method of Teaching on Biomedical Waste Management among the second year B Sc Nursing students Methodology: Quasi-experimental, Pretest Posttest Design was adapted. The study was conducted among 82 second-year B Sc Nursing students of PSG College of Nursing, Coimbatore. Based on the inclusion criteria, the study participants were divided for Experimental group I and Experimental group II. A convenient sampling technique was adopted. The questionnaire comprised 20 questions for assessing the knowledge of students on Biomedical Waste Management. Experimental group I was educated using Video Assisted Teaching and Experimental group II was educated using the Demonstration Method of Teaching. Descriptive and inferential statistics were used to analyze the data. Results: The mean and standard deviation of pre test and post test value for Video Assisted Teaching were 13.8 ± 2.12 and 16.39 ± 2.79 and for Demonstration Method of Teaching were 13.6 ± 2.10 and 15.92 ± 2.62. The calculated‘t’ value of Video Assisted Teaching was 6.03 and the Demonstration Method of Teaching was 3.35 which were more than tabulated value 2.02 at the level of p <0.05. There was a positive correlation (r = 0.715) between Video Assisted Teaching and Demonstration Method of Teaching among Nursing students on Bio-Medical Waste Management at 0.05 level (2-tailed). Conclusion: The finding of the study concluded that Demonstration Method of Teaching is effective than Video Assisted Teaching regarding knowledge on Bio-Medical Waste Management among second year B.Sc. Nursing students. Keywords: Evaluate, Effectiveness, Video-Assisted Teaching (VAT), Demonstration Method of Teaching (DMT), Knowledge on Bio-Medical Waste Management.